Sanger sequencing

Part One Sanger DNA Sequencing

Pyrosequencing-An Alternative to Traditional Sanger Sequencing

Problem statement: Pyrosequencing has the potential to rapidly and reliably sequence DNA taking advantages over traditional Sanger di-deoxy sequencing approach. Approach: A comprehensive review of the literature on the principles, applications, challenges and prospects of pyrosequencing was performed. Results: Pyrosequencing was a DNA sequencing technology based on the sequencing-by-synthesis p...

Sanger Sequencing White Paper a T C Aa T Factors Affecting Sanger Sequencing Robustness and Data Quality

In order to better understand the robustness of our protocols and to identify failure modes for Sanger Sequencing in the UMGC we carried out a series of experiments testing the effects of DNA template concentration, primer concentration, and sample purity on Sanger sequencing data quality. We determined that the UMGC’s Sanger sequencing processes are robust for a wide range of template and prim...

CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing

The advent of next generation gene editing technologies has revolutionized the fields of genome engineering in allowing the generation of gene knockout models and functional gene analysis. However, the screening of resultant clones remains challenging due to the simultaneous presence of different indels. Here, we present CRISP-ID, a web application which uses a unique algorithm for genotyping u...

Barcoding the food chain: from Sanger to high-throughput sequencing.

Society faces the complex challenge of supporting biodiversity and ecosystem functioning, while ensuring food security by providing safe traceable food through an ever-more-complex global food chain. The increase in human mobility brings the added threat of pests, parasites, and invaders that further complicate our agro-industrial efforts. DNA barcoding technologies allow researchers to identif...